The ChromaFlash™ Plant Chromatin Extraction Kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complexes from plants in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. It is also the recommended method for obtaining chromatin required by Epigentek’s one-hour ChIP method using the ChromaFlash™ One-Step ChIP Kit or the ChromaFlash™ One-Step Magnetic ChIP Kit. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays, or nuclear enzyme assays. - Extremely fast procedure: the entire procedure from plant samples to ready-to-use chromatin is less than 2 hours.
- Convenient and flexible: the kit is suitable for preparing both native chromatin and cross-linked chromatin from monolayer or suspension cells, or from tissues.
- Unsheared chromatin makes it customizable for various analysis workflows that require either intact or fragmented chromatin, including ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assays, etc.
Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous tool for studying protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the specific sequences of a gene in living cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) techniques. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated with a specific gene promoter region under various conditions can be achieved through a ChIP-PCR assay, while recruitment of meH3-K9 to the promoters on a genome-wide scale can be detected by ChIP-chip. In particular, a ChIP method with specific antibodies directly against various transcriptional factors is widely in demand.
Principle & Procedure The ChromaFlash™ Plant Chromatin Extraction Kit contains all the reagents required for carrying out a successful chromatin extraction directly from plants. Cell membranes of the sample, with or without cross-linking, are broken down using the provided lysis buffer. Chromatin or DNA-protein complex is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and stored at the appropriate temperature. | 
 Fig. 1. Schematic procedure of the ChromaFlash™ Plant Chromatin Extraction Kit.
Fig. 2. ChIP analysis of tri-methyl H3-K27 enriched in AGAMOUS gene with chromatin extract prepared from 2-week-old icu2-1/icu2-1 seedlings using the ChromaFlash™ Plant Chromatin Extraction Kit.
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