PRODUCT NUMBER:

MW: 25,000 - 29,000
Extinction Coefficient: 1.28

Frozen in 20 mM Malonate, pH 5.5, 1 mM EDTA, 400 mM NaCl.

Storage: -70°C or lower

Buy Purified Native Human Cathepsin L (CatL), Human Liver.
Bulk Qty Available.

The most powerful of the lysosomal proteases. It has a higher specific activity than cathepsin B and H in the degradation of a variety of physiological protein substrates. The level of cathepsin L is a strong predictor of relapse and survival following treatment of a primary breast tumor.

Activity: Greater than 1 unit per mg of protein. One unit is defined as the amount of enzyme that hydrolyzes one micromole of Z-Phe-Arg-AFC per minute at 25oC using 400 mM Na Acetate, pH 5.5 with 4 mM EDTA and 8 mM DTT as the activation buffer in the presence of Brij.

Prepared from tissue shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

Athens Research & Technology products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.

Product Citation:
Armstrong, Andrea, Niklas Mattsson, Hanna Appelqvist, Camilla Janefjord, Linnea Sandin, Lotta Agholme, Bob Olsson et al. "Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimer’s Disease." Neuromolecular medicine (2013): 1-11
Miller, Bailey, Aaron J. Friedman, Hyukjae Choi, James Hogan, J. Andrew McCammon, Vivian Hook, and William H. Gerwick. "The Marine Cyanobacterial Metabolite Gallinamide A Is a Potent and Selective Inhibitor of Human Cathepsin L." Journal of natural products (2013).

References:
Mason, R.W., Green, G.D.J. and Barrett, A.J. 1985. Biochem. J. 226, 233.
Klijn et al. 1998. J. Clin. Onc.16, 1013.
Barrett, A.J. and Kirschke, H. 1981. Methods Enzoymol. 80, 535.
Tchope, J.R. et al. 1991. Biochem. Biophys. Acta. 1076. 149.