Caution

Hygroscopic

Packaging

1, 5 g in glass bottle

250 mg in glass bottle

Application

Sodium butyrate has been used:
• as a component in homogenization and immunoprecipitation buffer for HDAC inhibition in fly embryos and insect s2 cells.
• as histone deacetylase inhibitor in breast cancer cell line MDA-MB-231, analysed by cell viability assay and viral replication assay.
• to enhance the production of recombinant tissue-plasminogen activator (t-PA) in Chinese hamster ovary (CHO) cells in a bioreactor.

Biochem/physiol Actions

Sodium butyrate is a short chain fatty acid inhibits histone deacetylase (HDAC) in millimolar range. Decreases Ca²⁺ release from intracellular stores. Induces apoptosis in several cell lines. Sodium butyrate induces the production of recombinant protein in Chinese hamster ovary (CHO) cells and other human cell lines. Sodium butyrate affects the proteome and gene expression pathways in the cells. It represses cell cycle related genes and modifies the genes involved in cell metabolism and apoptosis.