The CD45 molecule is also known as the Leukocyte Common Antigen (LCA) or T200 antigen, and is comprised of different glycoproteins ranging from 180-240 kDa. Expression of CD45 is found on all hemopoietic cells, e.g. granulocytes, monocytes, macrophages and lymphocytes, except mature erythroid cells. Detection of the different isoforms can distinguish, for example, between naive T cells and memory T cells, which is of interest in patients with immunodeficiency and autoimmune diseases.
| Target |
CD45 |
| Reactivity |
Human, Monkey |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
FCM |
| Recommended dilutions |
FCM: 20 µl/1 million cells. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Human Peripheral Blood Leucocytes. |
| Purification |
Affinity Chromatography |
| Form |
Liquid |
| Isotype |
IgG1 |
| Conjugation |
Biotin |
| Specificity |
The CD45 (HI30) antibody recognizes the 180, 190, 205 and 220-kDa long single chain type I transmembrane glycoprotein called leukocyte common antigen (LCA). LCA is typically expressed at high levels on all nucleated hematopoietic cells including bone marrow cells, thymocytes, lymphocytes, monocytes, granulocytes, but not on mature erythrocytes, platelets and non-hematopoietic tissues and cells. CD45 is critically required for T and B cell antigen receptor-mediated activation. |
| Storage |
Aliquot and store in the dark at 2°C to 8°C. Avoid exposure to light. |
| Molecular Weight |
180-220 kDa |
| Swiss Prot |
P08575
|
| GeneID |
5788
|
| Enzyme Commission Number |
EC 3.1.3.4 |
| Buffer |
The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium Azide |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 2-3 weeks. |
| Note |
This product is for research use only. |