The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta.The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication.In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.Two transcript variants encoding the same protein have been found for this gene.Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
| Target |
Proliferating Cell Nuclear Antigen (PCNA) |
| Reactivity |
Human |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
ELISA, WB, IHC, FCM |
| Recommended dilutions |
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Purified recombinant fragment of human PCNA (AA: 53-196) expressed in E. coli. |
| Purification |
Purified antibody |
| Isotype |
IgG1 |
| Conjugation |
Unconjugated |
| Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Molecular Weight |
28.7 kDa |
| Swiss Prot |
P12004
|
| GeneID |
5111
|
| Gene Symbol |
PCNA |
| OMIM |
176740
|
| HGNC |
8729 |
| Ensembl |
ENSG00000132646 |
| Concentration |
1.0 mg/ml |
| Buffer |
PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |