SelecTEV™ Protease is a 27 kD improved form of Tobacco Etch Virus (TEV) protease that has been engineered to be more stable, active, and specific than the native protease

SelecTEV™ Protease can be used with the Expresso® Solubility and Expression Screening System vectors, which contain a TEV cleavage recognition site.

For research use only. Not for human or diagnostic use.

SelecTEV™ Protease is a cysteine protease that recognizes the TEV recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly and cleaves between Gln and Gly. This seven-amino-acid sequence is rarely found in proteins, making SelecTEV™ Protease an ideal choice for tag removal of fusion proteins. The optimal temperature for cleavage is 30 °C, however the enzyme is active over a wide temperature range (4 – 30 °C) and pH (6.0 – 8.5) to accommodate your specific protein. Following digestion, SelecTEV™ Protease is easily removed from the cleavage reaction by affinity chromatography using the polyhistidine tag at the N-terminus of the protease. SelecTEV™ Protease is purified from E. coli by affinity chromatography using the polyhistidine tag and is 90% pure when visualized on an SDS-PAGE gel.