Lucigen’s E

Lucigen offers the following choices of strain: 1) E. cloni 10G Competent Cells: Routine cloning, subcloning, and plasmid isolation with or without blue/white screening. 2) E. cloni 10GF′ Competent Cells: Contain the F′ plasmid for infection with M13 to produce ssDNA.

And the following choices of efficiency: 1) E. cloni 10G Chemically Competent Cells (> 1 × 10^9 cfu/µg pUC DNA; (> 1 × 10^8 in 96-well plates). Highly efficient competent cells for routine cloning applications. Available in one transformation per tube (SOLOs) or two transformations per tube (DUOs). Also available in 96-well plates (which can be divided into 24-well seqments) for higher throughput. 2) E. cloni 10GF´ Chemically Competent Cells (≥5 × 10^8 cfu/µg pUC DNA). An excellent choice for producing single-stranded DNA for sequencing, mutagenesis, etc. 3) E. cloni 10G Chemically Competent Cells, Subcloning Grade (≥1 × 10^6 cfu/µg pUC DNA). The best value available anywhere for simple cloning and plasmid propagation.

cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use-only common laboratory equipment is required. These cells provide solutions for a wide range of applications at unparalled economical prices.