CYBRFast 1-Step RT-qPCR Lo-ROX Kit contains everything required for efficient cDNA synthesis and qPCR in a single tube using real-time instruments that support normalization with a standard ROX passive reference dye at a final concentration of 50 nM

CYBRFast GREEN DNA intercalating dye does not inhibit PCR, resulting in better fluorescent signals than other dyes, and is optimized for use with CYBRFast™ Hot Start Taq DNA Polymerase. Novel hot start technology prevents primer dimer formation and non-specific amplification resulting in higher yields and specificity. 1-Step Kits include a proprietary M-MLV Reverse Transcriptase (RT) molecule blended with an optimized RNase inhibitor, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Our M-MLV RT is ultra-stable and highly active, working on varied and difficult templates.

Convenient and reliable, CYBRFast 1-Step RT-qPCR Lo-ROX Kit is ideal for the sensitive and efficient quantification of RNA templates, including low copy number targets, under standard or fast cycling conditions - Please refer to our Instrument Compatibility Guide to ensure you have the correct ROX concentration for your system.

This approach offers simplicity and convenience and minimizes the possibility for contamination.