Cell stimulation cocktail is a ready to use cocktail that can be used for polyclonal cell activation. It is composed of the phorbol ester, PMA (Phorbol 12-Myristate 13-Acetate), a calcium ionophore (Ionomycin), and the protein transport inhibitors Brefeldin A and Monensin. It can be used to elicit cytokine production and retention in a variety of cell types allowing for detection of intracellular protein by flow cytometry.

It has been quality-tested by treatment of mouse splenocytes for 6 to 18 hours followed by intracellular staining and flow cytometric analysis of IL-2 or Ifn-gamma. It should be used at 1X final concentration for cell stimulation. It is recommended to add the cocktail directly to the cell suspension at 2 uL/mL to obtain a 1X final concentration.

Application: Flow cytometry and functional assays.