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Product Name | Mouse IgG2b Isotype Control (NCG2B.01) |
Description | Mouse IgG2b Isotype Control (NCG2B.01). The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype con |
Size | 500 µL |
Concentration | n/a |
Applications | Control: Assay-Dependent, ELISA: Assay Dependent, Flow Cytometry: 0.5 µg/test, Gel Shift: Assay Dependent, Immunofluorescence: Assay Dependent, Immunohistochemistry (Frozen): Assay Dependent, Immunohistochemistry (Paraffin): Assay Dependent, Immunoprecipitation: Assay Dependent, Western Blot: Assay Dependent |
Other Names | IgG2; IgG2b |
Gene, Accession, CAS # | n/a |
Catalog # | MA5-14447 |
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Order / More Info | Mouse IgG2b Isotype Control (NCG2B.01) from INVITROGEN ANTIBODIES |
Product Specific References | PubMed: 28317943 |
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