| 产品详情 |
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| Antigenic Specificity | LC3B |
| Clone | polyclonal |
| Host Species | Rabbit |
| Reactive Species | human, mouse, rat, porcine, avian, bacteria, bovine, canine, invertebrate, monkey, primate, golden Syrian hamster, zebrafish |
| Isotype | n/a |
| Format | immunogen affinity purified |
| Size | 0.1 ml |
| Concentration | 1.0 mg/ml |
| Applications | Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Proximity Ligation Assay, Chromatin Immunoprecipitation (ChIP), Knockout Validated. This LC3B antibody is tested for ICC/IF, IHC on paraffin embedded sections, IP and Western blot. Use in IHC on frozen sections reported in scientific literature (PMID: 20008275). Use in immunoblotting reported in scientific literature (PMID 28559895). Use in ELISA reported in scientific literature (PMID 20503249). Use in ChIP reported in scientific literature (PMID28431247). Use in FLOW reported in scientific literature (PMID: 27980456). In Western blot, bands are seen at 17 and 19 kDa corresponding to LC3-II and LC3-I. In some cases a non-specific band is seen at 21 kDa in mouse protein. In ICC/IF, cytoplasmic staining was observed in HeLa cells. Use in Proximity Ligation Assay reported in scientific literature (PMID 27219062). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. |
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
| Description | The LC3B Antibody from Novus Biologicals is a rabbit polyclonal antibody to LC3B. This antibody reacts with human, mouse, rat, porcine, avian, bacteria, bovine, canine, invertebrate, monkey, primate, golden syrian hamster, zebrafish. The LC3B Antibody has been validated for the following applications: Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Proximity Ligation Assay, Chromatin Immunoprecipitation (ChIP), Knockout Validated. |
| Immunogen | A synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100). [UniProt# Q9GZQ8] |
| Other Names | anti-LC3B, atg8f , Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, lc3b autophagy marker, LC3B ihc, LC3B immunohistochemistry, LC3B immunoprecipitation, LC3B western blot, lc3-i, ii, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, map1lc3b, microtubule associated protein 3 b, microtubule associated protein 3 beta, microtubule-associated protein 1 light chain 3 betaMAP1A/MAP1B light chain 3 B, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B |
| Gene, Accession # | MAP1LC3B, Gene ID: 81631, SwissProt: Q9GZQ8 |
| Catalog # | NB100-2220 |
| Price | |
| Order / More Info | LC3B Antibody from NOVUS BIOLOGICALS, LLC |
| Product Specific References | PubMed: 17913701, 17921520, 18188005, 18281291, 18391949, 18502787, 18713860, 18838554, 19017649, 19047137, 19060920, 19074461, 19098111, 19141455, 19182529, 19185734, 19216903, 19246570, 19265175, 19339495, 19370058, 19380511, 19433452, 19550143, 19622773, 19666601, 19700765, 19750228, 19786831, 19786832, 19786835, 19793721, 19861440, 19901560, 19920197, 19934334, 20008275, 20149359, 20162625, 20215500, 20348093, 20383065, 20404487, 20424262, 20439739, 20453062, 20454448, 20503249, 20808909, 20814235, 20837911, 20930077, 20946101, 21068542, 21080439, 21186265, 21187718, 21209089, 21225866, 21228817, 21253613, 21307647, 21353614, 21360721, 21433059, 21454445, 21459442, 21460622, 21467033, 21494837, 21535939, 21536669, 21545732, 21552986, 21555592, 21559479, 21576371, 21632946, 21641551, 21672589, 21710541, 21733849, 21738008, 21750112, 21768100, 21784250, 21784844, 21795851, 21842312, 21883444, 21920942, 21943220, 21944908, 21964292, 22015590, 22025610, 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