| 产品详情 |
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| Antigenic Specificity | IL-1 beta/IL-1F2 |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human, mouse, rat, porcine, bacteria, canine, primate |
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| Isotype | n/a |
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| Format | IgG purified, no preservative |
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| Size | 0.1 mg |
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| Concentration | n/a |
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| Applications | Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Electron Microscopy. Anti-Human IL-1B has been tested for use in neutralizations, ELISA, radioimmunoassays, flow cytometry, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17,000 MW mature IL-1B. For immunoblots, typically, IL-1B is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG to reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL-1B. For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of human and primate IL-1B activity in bioassays. It does not neutralize the biological activity IL-1a. It does not neutralize the biological activity of murine, rat or rabbit IL-1B. For neutralization, it is recommended to incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F(c) domain of the rabbit IgG molecule may interact with cells non-specifically. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The IL-1 beta/IL-1F2 Antibody from Novus Biologicals is a rabbit polyclonal antibody to IL-1 beta/IL-1F2. This antibody reacts with human, mouse, rat, porcine, bacteria, canine, primate. The IL-1 beta/IL-1F2 Antibody has been validated for the following applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Electron Microscopy. Detects IL-1 beta and does not cross react with IL-1 alpha |
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| Immunogen | This antibody was prepared by repeated immunizations with recombinant human IL-1 beta/IL-1F2 produced in E.coli. The MW of the recombinant 153 aa IL-1 beta was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1 beta converting enzyme (ICE, capase-1). |
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| Other Names | catabolin, IL-1, IL-1 beta, IL-1B, IL1-BETA, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta |
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| Gene, Accession # | IL1B, Gene ID: 3553, Accession: P01584, SwissProt: P01584 |
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| Catalog # | NB600-633 |
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| Price | |
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| Order / More Info | IL-1 beta/IL-1F2 Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | PubMed: 21217200, 21533109, 22898394, 23936223, 23959857, 25294684, 25916578, 26945693, 27321128, 27459270, 29131694, 29184853, 29311826, 29683202, 29892064, 30584238, 30799394, 30811968 |
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