| 产品详情 |
| Edit |   |
|---|
| Antigenic Specificity | IL-1 beta/IL-1F2 |
|---|
| Clone | polyclonal |
|---|
| Host Species | Rabbit |
|---|
| Reactive Species | mouse, rat |
|---|
| Isotype | n/a |
|---|
| Format | biotin conjugate |
|---|
| Size | 0.1 mg |
|---|
| Concentration | lyophilized |
|---|
| Applications | Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin. This antibody recognizes the 17,000 MW mature IL-1Beta . For immunoblots, typically, IL-1Beta is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells . PBMC are stimulated for 24 hours with 1% serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG - reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation - stain intracellular IL-1Beta . For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of mouse and rat IL-1Beta activity in bioassays. It does not neutralize the biological activity IL-1alpha. It does not neutralize the biological activity of human or primate IL-1Beta . For neutralization, it is recommended - incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used f. Caution should be exhibited as the F domain of the rabbit IgG molecule may interact with cells non-specifically.Flow Cytometry was reported in scientific literature. |
|---|
| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
|---|
| Description | The IL-1 beta/IL-1F2 Antibody [Biotin] from Novus Biologicals is a rabbit polyclonal antibody to IL-1 beta/IL-1F2. This antibody reacts with mouse, rat. The IL-1 beta/IL-1F2 Antibody [Biotin] has been validated for the following applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin. This antibody is primarily directed against mature, 17,000 MW mouse IL-1 beta and is useful in determining its presence in various assays |
|---|
| Immunogen | This antibody was prepared by repeated immunizations with recombinant mouse IL-1 beta/IL-1F2 produced in E.coli. The MW of recombinant mouse IL-1 beta was 17 kDa. |
|---|
| Other Names | catabolin, IL-1, IL-1 beta, IL-1B, IL1-BETA, IL1F2IL-1 beta, interleukin 1, beta, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta |
|---|
| Gene, Accession # | IL1B, Gene ID: 3553 |
|---|
| Catalog # | NBP1-42768 |
|---|
| Price | |
|---|
| Order / More Info | IL-1 beta/IL-1F2 Antibody from NOVUS BIOLOGICALS, LLC |
|---|
| Product Specific References | PubMed: 23494472 |
|---|
| 产品资料 |
|
|