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| Product Name | 25-OH Vitamin D3 HPLC Assay |
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| Description | For the determination of 25-OH vitamin D3 a sample preparation has to be performed. Therefore a precipitation step is combined with an extraction. After centrifugation the upper layer is injected into the HPLC system. The HPLC separation works with an isocratic method at 30 °C with a reversed phase column. Chromatograms are detected by an UV-detector. The separation takes 15 minutes for each run depending on the column used. Results are quantified by the delivered serum calibrator and calculated by the internal standard-method by integration of the peak areas or heights. Sample Types: serum, plasma. Time: 15 minutes • The Eagle Biosciences 25-OH Vitamin D3 HPLC Assay kit makes it possible to determine the vitamin in an easy, fast and precise method. The kit includes all reagents in ready to use form for preparation and separation of the samples with exception of the column (IC3401rp) and the controls (IC3401ko). Vitamin D is one of the most important essential bioregulators of the Ca2+- and phosphate metabolism in higher animals. Together with the peptide hormones parathormone and calcitonin it is responsible to obtain the Ca2+- and phosphate homoeostasis. The most important physiological functions of vitamin D are the stimulation of the intestinal resorption of Ca2+ and phosphate and their incorporation in the bone matrix. |
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| Size | 100 tests |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Vitamin D, 25-OH, 25 hydroxy, Cholecalciferol |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | VD331-H100 |
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| Order / More Info | 25-OH Vitamin D3 HPLC Assay from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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