| 产品详情 |
| Edit |   |
|---|
| Product Name | Recombinant Mouse DNA-dependent protein kinase catalytic subunit (Prkdc), partial |
|---|
| Description | Purity > 90% as determined by SDS-PAGE. MW: 34.6kDa. Partial: 3747-4015aa. N-terminal 6xHis-tagged. Mus musculus (Mouse). Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V (D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V (D)J recombination by activation of the hairpin endonuclease artis (DCLRE1C). The assbly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, C1D, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. |
|---|
| Size | 100ug |
|---|
| Concentration | n/a |
|---|
| Applications | RUO |
|---|
| Other Names | p460 |
|---|
| Gene, Accession, CAS # | Prkdc, UniProt: P97313 |
|---|
| Catalog # | RPC21856 |
|---|
| Price | |
|---|
| Order / More Info | Recombinant Mouse DNA-dependent protein kinase catalytic subunit (Prkdc), partial from BIOMATIK |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|