| 产品详情 |
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| Product Name | Catalase Activity Kit |
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| Description | The Catalase Activity Kit is designed to quantitatively measure catalase activity in a variety of samples. A bovine catalase standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in the provided Assay Buffer and added to the wells of a half area clear plate. Hydrogen peroxide is added to each well and the plate incubated at room temperature for 30 minutes. The supplied Colorimetric Detection Reagent is added, followed by diluted horseradish peroxidase and incubated at room temperature for 15 minutes. The HRP reacts with the substrate in the presence of hydrogen peroxide to convert the colorless substrate into a pink-colored product. The colored product is read at 560 nm. Increasing levels of catalase in the samples causes a decrease in H2O2 concentration and a reduction in pink product. The activity of the catalase in the sample is calculated after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of units of catalase activity per mL. Direct Enzyme Activity Assay. Intra Assay Precision: Three human serum samples diluted in Assay Buffer were run in replicates of 20 in an assay. The mean and precision of the calculated concentrations were: Sample 1- 1.71 U/mL, 3.5% CV Sample 2- 0.84 U/mL, 4.0% CV Sample 3- 0.48 U/mL, 4.8% CV Inter Assay Precision: Three human serum samples diluted in Assay Buffer were run in duplicates in twenty-one assays run over multiple days by three operators. The mean and precision of the calculated concentrations were: Sample 1- 1.79 U/mL, 11.9% CV Sample 2- 0.94 U/mL, 9.8% CV Sample 3- 0.53 U/mL, 12.3% CV. Range: 0.156 - 5 U/ml. Sensitivity: 0.052 U/ml. Time: 45 minutes. |
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| Size | 2 x 96 well |
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| Concentration | n/a |
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| Applications | Colorimetric assay used to quantitatively measure catalase activity in a variety of samples. RUO |
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| Other Names | StressXpress® Cas1 Activity Kit, CAT Activity Kit, Cs1 Activity Kit, MGC138422 Activity Kit, MGC138424 Activity Kit |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | SKT-215-192 |
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| Order / More Info | Catalase Activity Kit from STRESSMARQ BIOSCIENCES INC. |
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| Product Specific References | PubMed: 28235023 |
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