| 产品详情 |
| Edit |   |
|---|
| Product Name | ApaI |
|---|
| Description | 5'-G G G C C^C-3' 3'-C^C C G G G-5' Concentration: 10u/ul Source: Acetobacter pasteurianus Buffer: 10mM Tris-HCl (pH 7.5), 10mM MgCl2 and 0.1mg/ml BSA. Incubate at 37°C. (Incubation at 30°C results in a 2-fold increase in activity.) Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 50mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug lambda DNA x 16 hours) with ApaI. Ligation/Recutting Assay After 50-fold overdigestion (3u/ug DNA x 17 hours) with ApaI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.02uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligon |
|---|
| Size | 5000U |
|---|
| Concentration | n/a |
|---|
| Applications | n/a |
|---|
| Other Names | n/a |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | A2298-63 |
|---|
| Price | |
|---|
| Order / More Info | ApaI from UNITED STATES BIOLOGICAL |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|