| 产品详情 |
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| Product Name | Myelin Basic Protein, Dephosphorylated, Bovine (MBP) |
| Description | Purified from bovine brain using HPLC and de-phosphorylated using Lambda protein phosphatase. Lambda protein phosphatase was inactivated, in the presence of 10mM sodium EDTA, at 65° C for one hour. Purity was assessed at 95% using SDS-PAGE and Coomassie blue staining of 1ug of product. Developed for use in radioactive and non-radioactive kinase assays. In vitro, myelin basic protein (MBP) is a substrate for phosphorylation by several different protein kinases including MAPK, PKA, calmodulin-dependent protein kinase, PKC and phosphorylase kinase. Even highly specific protein kinases such as Raf1, MEK and MEKK can utilize MBP as an alternative substrate. Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact my |
| Size | 5mg |
| Concentration | n/a |
| Applications | WB |
| Other Names | n/a |
| Gene, Accession, CAS # | SwissProt: P02687 |
| Catalog # | M9758-06 |
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| Order / More Info | Myelin Basic Protein, Dephosphorylated, Bovine (MBP) from UNITED STATES BIOLOGICAL |
| Product Specific References | n/a |
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