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| Product Name | Avidin/Biotin Blocking Kit, BioAssay™ |
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| Description | Avidin/Biotin Blocking Kit BioAssay™ is designed to eliminate background caused by endogenous avidin or biotin-like substances. The kit contains 18ml each of avidin and biotin solutions. Kit Components: A4500-14A: Avidin Solution, 1x18ml. Supplied as a liquid, 0.05% sodium azide A4500-14B: Biotin Solution, 1x18ml. Supplied as a liquid, 0.05% sodium azide Protocol: These blocking steps should be performed before the primary antibody incubation step. 1. Apply 100ul (2 drops) of A4500-14A to the tissue. 2. Incubate for 10 minutes at RT. 3. Rinse extensively with PBS. 4. Apply 100ul (2 drops) of A4500-14B to the tissue. 5. Incubate for 10 minutes at RT. 6. Rinse extensively with PBS. The endogenous avidin/biotin activity is now blocked and the tissue section is ready for immunohistochemical staining. |
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| Size | 1 kit |
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| Concentration | n/a |
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| Applications | IHC |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | A4500-14 |
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| Order / More Info | Avidin/Biotin Blocking Kit, BioAssay™ from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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