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| Product Name | Purification Media, Nickel Beads, BioSeparation™ |
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| Description | Recombinant proteins tagged with 6-10 poly Histidines can be purified in one step by ion metal affinity chromatography (IMAC). Several metal ions exhibiting affinity to poly-Histidines are commonly used, Nickel and Cobalt being the most popular. Tagging the proteins may be done at the N-ter or the C-ter by cloning a gene in dedicated expression vectors encoding to the poly Histidines stretch, enabling the purification of the recombinant protein resulted. Nickel Beads are high quality Nickel affinity-resin product, that exhibit high capacity and good selectivity towards His-tagged proteins. Purification with Nickel Beads may be done in a variety of formats, such as gravity-flow columns and small or large-scale batches. The preferred purification strategy is to start by using the native purification conditions and only later on, if required, use denaturing conditions for proteins that are accumulated as inclusion bodies or in cases when the 6xHis purification tag is not exposed in the na |
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| Size | 5ml, 10ml |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | P9100-65 |
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| Order / More Info | Purification Media, Nickel Beads, BioSeparation™ from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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