| 产品详情 |
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| Antigenic Specificity | PCNA |
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| Clone | PC10 |
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| Host Species | Mouse |
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| Reactive Species | human, mouse, rat, porcine, chicken, Drosophila, fish, primate, rabbit, yeast, zebrafish |
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| Isotype | IgG2a kappa |
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| Format | Protein A purified |
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| Size | 0.1 ml |
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| Concentration | 1 mg/ml |
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| Applications | Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating. Use in Immunocytochemistry/Immunofluorescence has been reported in scientific literature (PMID: 22068968). It detects a band at 30 kDa by Western blot. For IHC on paraffin sections, heat-mediated citrate buffer antigen retrieval is recommended. ELISA was reported in scientific literature. Use in chromatin immunoprecipitation reported in scientific literature (PMID 26883631). Use in Immunohistochemistry-free floating reported in scientific literature (PMID 27212918). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | The PCNA Antibody (PC10) from Novus Biologicals is a mouse monoclonal antibody to PCNA. This antibody reacts with human, mouse, rat, porcine, chicken, drosophila, fish, primate, rabbit, yeast, zebrafish. The PCNA Antibody (PC10) has been validated for the following applications: Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating. Specific for PCNA p36 protein expressed at high levels in proliferating cells |
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| Immunogen | Protein A-rat PCNA fusion obtained from pC2T |
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| Other Names | cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen |
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| Gene, Accession # | PCNA, Gene ID: 5111, Accession: P12004, SwissProt: P12004 |
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| Catalog # | NB500-106 |
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| Price | |
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| Order / More Info | PCNA Antibody from NOVUS BIOLOGICALS, LLC |
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| Product Specific References | PubMed: 12405950, 1695635, 18570248, 20332601, 20370940, 21865299, 22068968, 22431921, 22828727, 22967007, 23451280, 23520190, 23658366, 24754742, 25152621, 25245017, 26298564, 26883631, 27046485, 27212918, 27792753, 28088007, 28703800, 29725291, 30075024, 30295833, 30771486 |
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