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Product Name | IgG Allergy (22 Foods) ELISA Assay Kit |
Description | Diluted serum samples are incubated with antigen extracts from 22 different foods immobilised on microtitre wells. After washing away unbound serum components, goat anti-human IgG conjugated to horseradish peroxidase is added to the wells, and this binds to surface-bound antibodies in the second incubation. Unbound conjugate is removed by washing, and a solution containing 3,3',5,5'-tetramethylbenzidine (TMB) and enzyme substrate is added to trace specific antibody binding. Addition of Stop Solution terminates the reaction and provides the appropriate pH for colour development. The optical densities of the standards, positive control and samples are measured using a microplate reader at 450nm. Sample Types: serum, plasma. Range: Negative <8 AU/ml, Borderline 8-12.5 AU/ml, Positive 12.5+. Sensitivity: Cut-off. Time: 1.25 hours • Many people exhibit chronic food sensitivity reactions to specific food antigens. Unlike the immediate effects of IgE-mediated allergy, IgG-mediated food sensitivity reactions may take several days to appear. Controlled removal of the problem foods from the patient's diet will, in many cases, rapidly improve the patient's condition. General lethargy, weight gain, dermatitis, arthritis and tiredness are associated with food allergies. Irritable bowel syndrome may also be linked to food sensitivity. |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | IgG, food allergy |
Gene, Accession, CAS # | n/a |
Catalog # | CNS129 |
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Order / More Info | IgG Allergy (22 Foods) ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |
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