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Antigenic Specificity | mCherry |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | n/a |
Isotype | n/a |
Format | immunoaffinity purified |
Size | 100 µg |
Concentration | 1.1 mg/mL |
Applications | ELISA, Immunohistochemistry, IF Microscopy, Western Blot. Polyclonal anti-mCherry is designed to detect mCherry, RFP, and its variants. Anti-mCherry (Discosoma sp.) are intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS). Researchers should determine optimal titers for applications that are not stated. In addition, we performed conjugation of RFP antibodies to either fluorescent dyes, biotin or horseradish peroxidase to further facilitate RFP protein detection and quantification. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | mCherry was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against mCherry, RFP and its variants: tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified mCherry. No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal. |
Immunogen | The immunogen is a mCherry mutant varient fusion protein of RFP corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma. |
Other Names | rabbit anti-mCherry antibody, RFP, mCherry monomeric red fluorescent protein, Red Fluorescent Protein (RFP), rDsRed, Discosoma sp. Red Fluorescent Protein, mRFP1, mBanana, mHoneydew, mPlum, mOrange, mStrawberry, mTangerine |
Gene, Accession # | DsRed |
Catalog # | 600-401-P16 |
Price | |
Order / More Info | mCherry Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | Valley, M. T., Henderson, L. G., Inverso, S. A., & Lledo, P. M. (2013). Adult neurogenesis produces neurons with unique GABAergic synapses in the olfactory bulb. The Journal of Neuroscience, 33(37), 14660-14665.Kao RM, Rurik JG, Farr GH 3rd, Dong XR, Majesky MW, Maves L. (2015) Pbx4 is Required for the Temporal Onset of Zebrafish Myocardial Differentiation. J Dev Biol. 2015;3(4):93-111. |
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