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| Product Name | Swine IL-10 ELISA KIT |
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| Description | The Eagle Biosciences Swine Interleukin 10 (IL-10) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique A monoclonal antibody specific for IL-10 has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps any IL-10 present is bound by the immobilized antibody and the detection antibody specific for IL-10 is binds to the combination of capture antibody-IL-10 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-10 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-10 standard dilutions and IL-10 sample concentration determined. Sample Types: serum, plasma, cell culture. Range: 15.625 - 1000 pg/ml. Sensitivity: 7 pg/ml. Time: 3.0 hours • Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 cytokine family. This family currently comprises IL-10, IL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1-4). All IL-10 family members are secreted α-helical proteins. Porcine IL-10 is a secreted, possibly glycosylated, polypeptide with an 18 kDa molecular weight (5). Based on human studies, porcine IL-10 is likely to circulate as a nondisulfide-linked homodimer (3). Porcine IL-10 is synthesized as a 175 amino acid (aa) precursor with an 18 aa signal sequence and a 157 aa mature form. The mature segment has one potential N-linked glycosylation site plus four cysteines which form two intrachain disulfide bridges (6). Mature porcine IL-10 shows 71%, 70%, 76%, 75%, 77%, and 71% aa sequence identity to rat (7), mouse (8), human (9), guinea pig (10), canine (11), and cotton rat (12) IL-10, respectively. Upon activation, mammalian cells known to secrete IL-10 include NK cells (13), cytotoxic CD8+ T cells secreting Th2-like cytokines (14), CD4+CD45RA- (memory) Th1 and Th2 cells (15), macrophages (16), monocytes (17), CD5+ and CD5- B cells (18, 19), dendritic cells (20, 21), hepatic stellate (Ito cells) (22), keratinocytes (23), melanoma cells (24), mast cells (25), placental cytotrophoblasts (26), and fetal erythroblasts (27). |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Interleukin 10, IL-10 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | L1051-K01 |
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| Order / More Info | Swine IL-10 ELISA KIT from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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