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| Product Name | Swine IL-2 ELISA KIT |
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| Description | The Eagle Biosciences Swine Interleukin 2 (IL-2) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-2 has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash step, any IL-2 present is bound by the immobilized antibody and the detection antibody specific for IL-2 is binds to the combination of capture antibody-IL-2 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-2 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-2 standard dilutions and IL-2 sample concentration determined. Sample Types: serum, plasma, cell culture. Range: 3.9 - 125 pg/ml. Sensitivity: 2 pg/ml. Time: 3.0 hours • Interleukin 2 (IL-2) is a pleiotropic cytokine produced primarily by mitogen- or Antigens-activated T lymphocytes (1, 2). Human IL-2 (also known as T-cell growth factor) is produced by T-cells in response to Antigensic or mitogenic stimulation. IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells promoting proliferation and maturation. IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendocytes. IL-2 is involved in treatment of cancers such as melanoma and renal cell cancer. It plays a key role in promoting the clonal expansion of Antigens-specific T cells. In addition, IL-2 has also been shown to mediate multiple immune responses on a variety of cell types. At the amino acid sequence level, there is approximately 72% similarity between mature porcine and human IL-2. The biological effects of IL-2 are mediated by specific cell surface receptor complexes. The functional high-affinity receptor for IL-2 is composed of three distinct polypeptide chains (3, 4). IL-2 stimulates the proliferation of thymocytes; stimulates the proliferation and differentiation of activated B cells; promotes the growth, differentiation and cytocidal activity of monocytes; induces the growth of natural killer cells and stimulates cytokine production by these cells as well as the cytolytic activity of these cells; enhances the production of lymphocyte-activated killer (LAK) cells; and induces the proliferation and differentiation of oligodendrocytes (1, 2). |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Interleukin 2, IL-2 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | IL251-K01 |
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| Order / More Info | Swine IL-2 ELISA KIT from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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