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| Product Name | Active Recombinant Human EGF |
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| Description | Human recombinant protein expressed in Nicotiana benthamiana. It is produced by transient expression in non-transgenic plants. This product contains no animal-derived components or impurities. Animal Free product. Human Recombinant Epidermal Growth Factor, (rh EGF) is a is a small mitogenic polypeptide which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids. EGF stimulates the proliferation and differentiation of epithelial cells from skin, cornea, lung and tracheal tissue and the gastrointestinal tract. EGF also promotes growth and migration of keratinocytes and enhances the proliferation of fibroblasts and embryonic cells. It is a member of a growth factor family which is characterized by the presence of 6 conserved cysteine motifs that form three disulphide bonds. The biological effects of EGF are mediated by a specific transmembrane receptor (EGF-R). The binding of EGF to EGFR will induce receptor dimerization, which is required for activating the tyrosine kinase in the receptor cytoplasmic domain. Thus, EGF triggers several signal transduction pathways including JAK/STAT, Ras/ERK and PI3K/AKT pathways. EGF plays an important role in wound healing and organogenesis .In addition to its proliferative effects, it participle of a variety of other bioactivities, including effects on cytoskeletal organization, cell migration and the synthesis and turnover of extracellular matrix molecules. Therefore, hEGF has wide application prospects in clinical and cosmetic fields. Recombinant full length EGF is a 7 kDa globular protein containing 54 amino acid residues (amino acids 971 al 1023 P01133 (EGF_HUMAN) with His tag at N-terminal. Purity >97% by SDS-PAGE gel |
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| Size | 1 µg, 10 µg, 100 µg, 250 µg, 500µg |
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| Concentration | n/a |
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| Applications | Functional studies, Cell assay, SDS-PAGE, Western Blot, Antibody Production. EGF is commonly used as a supplement in serum-free or reduced serum media used for culture of mammalian cells. |
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| Other Names | Urogastrone |
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| Gene, Accession, CAS # | UniProt: P01133 |
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| Catalog # | RF0102 |
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| Order / More Info | Active Recombinant Human EGF from AGRENVEC |
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| Product Specific References | - Carpenter, G. and Cohen, S., 1979. Epidermal Growth Factor. Annu. Rev. Biochem., 48, 193-216. - Brown, G. L. et al., 1989. Enhancement of wound healing by topical treatment with epidermal growth factor. N. Engl. J. Med., 321(2):76-9. - Alemdaroglu, C. et al., 2006. An investigation on burn wound healing in rats with chitosan gel formulation containing epidermal growth factor. Burns. 32(3):319-27. - Burgess, A. W., 2008. EGFR family: Structure physiology signalling and therapeutic targets. Growth Factors. Vol. 26, No. 5, Pages 263-274. - Heo, J. S. et al., 2006. EGF stimulates proliferation of mouse embryonic stem cells: involvement of Ca2+ influx and p44/42 MAPKsAm. J. Physiol. Cell. Physiol., 290:C123. - Kitazawa, T. 1990. The mechanism of accelerated corneal epithelial healing by human EGF. Invest Ophthalmol. Vis. Sci., 31(9):1773-7. - Henson, E. S. and Gibson, S. B., 2006. Surviving cell death through epidermal growth factor (EGF) signal transduction pathways: implication for cancer therapy. Cell Signal, Dec;18 (12), 2089-97. - Rubin, J.S., et al., 1991. A broad-spectrum human lung fibroblast-derived mitogen is a variant of hepatocyte growth factor Proc. Natl. Acad. Sci. USA. Vol. 88, pp. 415-419. |
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