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Product Name | 1,25-Dihydroxy Vitamin D RIA |
Description | The IDS 1,25-Dihydroxy Vitamin D RIA kit is a complete assay system for the purification of 1,25D in patient samples by immunoextraction followed by quantitation by 125I RIA. Patient samples are delipidated and 1,25D extracted from potential cross-reactants by incubation for 3 hours with a highly specific solid phase monoclonal anti-1,25D. The immunoextraction gel is then washed and purified 1,25D eluted directly into glass assay tubes. Reconstituted eluates and calibrators are incubated overnight with a highly specific sheep anti-1,25D. 125I 1,25D is added and incubation continued for 1 hrs. Separation of bound from free is achieved by a short incubation with Sac-Cel® followed by centrifugation, decantation and counting. Bound radioactivity is inversely proportional to the concentration of 1,25D. |
Size | n/a |
Concentration | n/a |
Applications | RIA |
Other Names | 1,25-OHD, 1,25-dihydroxyvitamin D, calcitriol kit, assay, immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | AA-54F1/AA-54F2 |
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Order / More Info | 1,25-Dihydroxy Vitamin D RIA from IMMUNODIAGNOSTIC SYSTEMS |
Product Specific References | n/a |
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