产品名称 Annexin V-Cy3 Apoptosis Kit Plus from CREATIVE BIOMART
产品货号 Kit-2356
产品价格 现货询价,电话:010-67529703
产品规格 n/a
产品品牌 CREATIVE BIOMART
产品概述
产品详情
Edit&nbsp
Product NameAnnexin V-Cy3 Apoptosis Kit Plus
DescriptionThe assay is based on the observation that soon after initiating apoptosis, cells translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a strong natural affinity for PS. The one-step staining procedure takes only 10 minutes. In addition, the assay can be directly performed on; Live cells, without the need of fixation. The Annexin V-Cy3 Apoptosis Detection Kit Plus includes annexin V-Cy3, SYTOX green dye, and binding buffer. The SYTOX green dye is impermeant to; Live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence by binding to cellular nucleic acids. After staining a cell population with annexin V-Cy3 and SYTOX Green dye in the provided binding buffer, apoptotic cells show red fluorescence, dead cells show green fluorescence and; Live cells show little or no fluorescence. These populations can easily be distinguished by Fluorescence microscopy using FITC and rhodamine filters or by flow cytometry using the FL1 channel (Ex. 488 nm/Em. 530 nm) for SYTOX Green dye and FL2 channel for Annexin V-Cy3 (Ex. 543 nm/Em. 570 nm).
Sizen/a
Concentrationn/a
ApplicationsDetecting apoptosis in living cells by flow cytometry or fluorescence microscopy. Cy3 shows brighter red fluorescence.
Other Namesn/a
Gene, Accession, CAS #n/a
Catalog #Kit-2356
Price
Order / More InfoAnnexin V-Cy3 Apoptosis Kit Plus from CREATIVE BIOMART
Product Specific Referencesn/a
产品资料