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| Product Name | Alpha 1 Antitrypsin ELISA |
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| Description | The Alpha-1 Antitrypsin ELISA assay determines human alpha-1-antitrypsin according to the ``sandwich''-principle. Alpha-1 Antitrypsin in sample, standard and controls binds to antibodies, which are coated to the microtiter plate. After a washing step a peroxidase labeled detection antibody is added. A second washing step is followed by the addition of the substrate which is converted to a colored product by the peroxidase. The reaction is terminated by the addition of an acidic stop solution. The optical densities are read at 450 nm (against the reference wavelength 620 nm) in a microtiter plate reader. The α-1-AT concentration can be calculated from the standard curve. Sample Types: stool, serum, plasma. Range: 3.3 - 90 ng/ml. Sensitivity: 1.5 ng/ml. Time: 2 hours • Alpha-1-Antitrypsin is a 52 kD glycoprotein, which is produced by the liver, intestinal macrophages, monocytes and mucous membrane cells of the gut. It belongs to the group of acute phase proteins and is one of the most important proteinase inhibitors. Alpha-1-antitrypsin inhibits, beside others, the proteinases trypsin and the elastase of neutrophils. A lack of α-1-AT leads to an enhanced proteolysis. Only a very small amount of alpha-1-antitrypsin is cleaved or resorbed in the gut. Therefore the measurement of α-1-AT in stool reflects the permeability of the gut during inflammatory processes. The Eagle Biosciences Alpha-1 Antitrypsin ELISA Kit allows an easy, rapid and precise quantitative determination of alpha-1-antitrypsin in biological samples. The kit includes all reagents ready to use for preparation of the samples. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Alpha 1 Antitrypsin, a1AT, serum trypsin inhibitor, alpha-1 proteinase inhibitor, A1PI |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | A1A35-K01 |
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| Order / More Info | Alpha 1 Antitrypsin ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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