| 产品详情 |
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| Product Name | ANA screen (Antinuclear Antibody) ELISA |
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| Description | The Eagle Biosciences ANAscreen ELISA Assay Kit is an enzyme immunoassay for the semi-quantitative determination of IgG antibodies to nuclear and cytoplasmic Antigenss. Antibodies of the controls and diluted patient samples react with nuclear and cytoplasmic Antigenss immobilized on the solid phase of microtiter plates. Use of complete HeLa nuclei enriched with recombinant and native Antigenss guarantees the specific binding of autoimmune antibodies of the specimen under investigation. Following an incubation period of 60 min at room temperature (RT, 18...25°C), unbound sample components are removed by a wash step. The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP). Within the incubation period of 30 min at RT, excessive conjugate is separated from the solid-phase immune complexes by the following wash step. HRP converts the colorless substrate solution of 3,3',5,5'-tetramethyl benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispnsing an acidic solution into the wells after 15 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. Patient ratios are calculated by dividing the respective OD of the specimen with the calculated cut-off OD. Sample Types: serum, plasma. Range: Cut-off. Sensitivity: Cut Off Control. Time: 2.5 hours • Systemic autoimmune diseases such as systemic lupus erythematosus, scleroderma, rheumatoid arthritis, Sjoegren's syndrome, dermatomyositis, mixed connective tissue disease are characterized by the appearance of a variety of autoantibodies directed against components of the cell nucleus. Although significance and pathological relevance of some auto-antibodies are not completely revealed yet, the detection of auto-antibodies is widely established and plays an important role in the diagnosis of systemic autoimmune diseases (1,2,3). ANAscreen allows the detection of total autoantibodies to nuclear and cytoplasmic Antigenss in one sample as a summary parameter in the diagnosis of systemic autoimmune disorders. Antigensic combination of complete HeLa nuclei with recombinant proteins and purified native Antigenss guarantees a maximum of sensitivy and specificity for the ANA detection. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | ASSAY EIA ELISA IMMUNOASSAY KIT ANA screen Antinuclear Antibody TEST ANS31-K01, auto immune |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ANS31-K01 |
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| Order / More Info | ANA screen (Antinuclear Antibody) ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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