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| Product Name | Anti-β2 GP-I (Beta 2 Glycoprotein 1) ELISA |
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| Description | The Eagle Biosciences Anti- β2 GP-I ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of IgG or IgM antibodies to β2 glycoprotein-I in human serum or plasma. The antibodies of the standards, the positive control and diluted patient samples react with the human β2 GP-I, immobilized on the solid phase of microtiter plates. The use of highly purified β2 GP-I guarantees the specific binding of antibodies to β2 glycoprotein-I of the specimen under investigation. Following an incubation period of 60 min at room temperature (RT), unbound serum components are removed by a wash step.The bound antibodies react specifically with anti-human-IgG or IgM conjugated to horseradish peroxidase (HRP) within the next incubation period of 30 min at RT. Excessive conjugate is separated from the solid-phase immune complexes by an additional wash step. HRP converts the colorless substrate solution of 3,3`,5,5`-tetramethyl-benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an added acidic solution (H2SO4) into the wells, after 15 min at RT turning the solution from blue to yellow.The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specifically bound antibodies.The standard curve is established by plotting the antibody concentrations of the standards (x-axis) and their corresponding OD-values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve. Sample Types: serum, plasma. Range: 1-300 U/mL. Sensitivity: 1 U/ml. Time: 2.5 hours • APAS is an autoimmune disorder comprising such clinical symptoms like arterial or venous thrombosis, thrombocytopenia and recurrent fetal loss. Primary APAS as well as systemic lupus erythematosus (SLE) are characterized by the appearance of autoantibodies to negatively charged phospholipids (1). Although significance and pathological relevance of phospholipid anti-bodies are not completely revealed yet, the detection of several autoantibody specificities is usually applied to the differential diagnosis and follow-up of systemic rheumatic inflammatory diseases.Unlike phospholipid antibodies which occur in some patients having infectious disease, phospholipid antibodies of autoimmune disease patients seem to recognize the relevant phospholipids in association with a plasma protein cofactor. One of these cofactors has been identified as b2 glycoprotein-I (b2 GP-I) (apo lipo pro tein H) (2,3). b2 GP-I, a serum protein with a molecular weight of 50 kDa affects platelet aggregation and coagulation.The positively charged fifth domain of b2 GP-I interacts with negatively charged phospholipids or activated polystyrol surfaces of ELISA wells. This interaction results in conformational changes of b2 GP-I and the creation of new epitopes apparently recognized by autoimmune phospholipid autoantibodies. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | B2G31-K01 ASSAY EIA ELISA IMMUNOASSAY KIT Anti Beta Glycoprotein 1 GP1,APAS, anti phospholipid |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | B2G31-K01 |
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| Order / More Info | Anti-β2 GP-I (Beta 2 Glycoprotein 1) ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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