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Product Name | Anti-Phospholipid Screen IgG/IgM ELISA Kit |
Description | An enzyme immunoassay test system to screen for the presence of IgG and IgM class autoantibodies against cardiolipin, phosphatidyl serine, phosphatidyl inositol, phosphatidic acid and beta-2-glycoprotein I in human serum or plasma. A mixture of highly purified cardiolipin, phosphatidyl serine, phosphatidyl inositol, phosphatidic acid and human beta-2-Glycoprotein I is bound to microwells. The determination is based on an indirect enzyme linked immune reaction with the following steps:Specific antibodies in the patient sample bind to the antigen coated on the surface of the reaction wells. After incubation, a washing step removes unbound and unspecifically bound serum or plasma components. Subsequently added enzyme conjugate binds to the immobilized antibody-antigen-complexes. After incubation, a second washing step removes unbound enzyme conjugate. After addition of substrate solution the bound enzyme conjugate hydrolyses the substrate forming a blue coloured product. Addition of an acid stops the reaction generating a yellow end-product. The intensity of the yellow color correlates with the concentration of the antibody-antigen-complex and can be measured photometrically at 450 nm. |
Size | 96 wells |
Concentration | n/a |
Applications | n/a |
Other Names | EIA, enzyme immunoassay, assay |
Gene, Accession, CAS # | n/a |
Catalog # | EIA3591 |
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Order / More Info | Anti-Phospholipid Screen IgG/IgM ELISA Kit from DRG INTERNATIONAL INC. |
Product Specific References | n/a |
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