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Product Name | Chicken IGF-I, recombinant protein (Receptor Grade) |
Description | Product Specifications Chicken IGF-I: The Product: Chicken IGF-I is a polypeptide comprising 70 amino acids, eight of which differ from the amino acid sequence of human IGF-I. Chicken IGF-I has been shown to stimulate growth in many cell types, including rat L6 myoblasts and chicken embryo fibroblasts, hepatocytes,adipocytes and myoblasts. Link to Sequence: Source: Chicken IGF-I is produced recombinantly in E. coli using a patented expression system. The chicken IGF-I molecule is correctly folded and several chromatography purification steps are employed to yield Receptor Grade chicken IGF-I.Purity: > 95% (as determined by HPLC and N-terminal sequence analysis)Molecular Weight: 7738 daltons - confirmed by Mass SpectrometryN-terminal sequence analysis of recombinant product: 5 residues >95% single sequence.Biological Activity: (stimulation of protein synthesis in rat L6 myoblasts) ED50 <30 ng/mlEndotoxin (LAL): <0.1 EU/ugStorage/Stability: At least 2 years at 2-4° C (lyophilised)Appearance: White PowderState: Dried from 0.1 M acetic acid: under dry nitrogen @ slight vacuum (-25kPa)Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g. <1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles. |
Size | 20 µg |
Concentration | n/a |
Applications | n/a |
Other Names | Igf-1, insulin-like growth factor-1, Somatomedin c, ILGF1, Mechano growth factor |
Gene, Accession, CAS # | n/a |
Catalog # | HU020 HU100 HM010 |
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Order / More Info | Chicken IGF-I, recombinant protein (Receptor Grade) from IMMUNOLOGICAL & BIOCHEMICAL TESTSYSTEMS GmbH |
Product Specific References | Ongabesan et al:BMPs and BMPRs in chicken ovary and the effects of BMP-4 and -7 on granulosa cell proliferation and progesterone production in vitro. Am J Physiol Endocrinol Metab (July 29, 2003). 10.1152/ajpendo.00104.2003. Link to article.This is a GroPep specification. ibt is authorized distributor of GroPep. |
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