产品详情 |
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Antigenic Specificity | 5-HIAA (5-Hydroxyindoleacetic Acid) |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | rat, sea slug |
Isotype | n/a |
Format | serum |
Size | 100 µL |
Concentration | n/a |
Applications | Immunohistochemistry, Immunocytochemistry, Immunofluoresence |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | The 5-HIAA antibody was raised to 5-HIAA coupled to BSA with paraformaldehyde. The antibody produces moderate labeling of raphe neurons in normal rat. In rats whose serotonergic system has been activated, staining intensity is increased to a maximum label. Recommended dilutions of the antiserum are 1/200-1/400 for indirect immunofluorescence and 1/4000-1/8000 for biotin-streptavidin/HRP technique. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Schipper and Tilders, 1983). Results showed that the 5-HIAA antibody dose dependently stained 5-HIAA but did not stain any concentration of 5-HT or 5-HTP. The antiserum was also tested by pre-adsorption at 25 µg/mL with various BSA conjugates. While pre-adsorption with 5-HIAA conjugate completely eliminates immunolabeling, pre-adsorption with conjugates of 5-HT,5-HTP and dopamine had no effect on staining intensity or distribution of stain. |
Immunogen | 5-HIAA |
Other Names | 5-HTT, 5-HTTLPR, 5HTT, HTT, OCD1, SERT, hSER |
Gene, Accession # | SLC6A4, Gene ID: 6532 |
Catalog # | 24274 |
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Order / More Info | 5-HIAA (5-Hydroxyindoleacetic Acid) Antibody from IMMUNOSTAR INC. |
Product Specific References | PubMed: 16219029, 16110094, 2732954, 24657391 |
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