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Antigenic Specificity | 5-HTP (5-Hydroxytryptophan) |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | rat, sea slug (aplysia californica, tritonia diomedea |
Isotype | n/a |
Format | serum |
Size | 100 µL |
Concentration | n/a |
Applications | Immunohistochemistry, Immunocytochemistry, Immunofluoresence, Western Blot |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | The 5-HT Antibody was raised in rabbit against 5-HTP coupled to BSA with paraformaldehyde. The antibody has a proven maximum biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat raphe nuclei. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Shipper and Tilders, 1983). Results showed that the 5-HTP antibody dose dependently stained 5-HTP but did not stain any concentration of 5-HT or 5-HIAA. The antiserum was also tested by pre-adsorption with indole/paraformaldehyde/BSA conjugates. Staining was completely blocked by pre-adsorption with 5-HTP conjugate and unaffected by 5-HIAA or 5-HT conjugate. |
Immunogen | 5-HTP |
Other Names | DI, Va |
Gene, Accession # | Avp, Gene ID: 24221 |
Catalog # | 24446 |
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Order / More Info | 5-HTP (5-Hydroxytryptophan) Antibody from IMMUNOSTAR INC. |
Product Specific References | PubMed: 22179106, 21296106, 11477599 |
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