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Product Name | AKT3 Human Recombinant Protein |
Description | Recombinant protein corresponds to amino acids 1 to 481 of mature human AKT3; Akt isoform 3. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC. |
Size | 10 µg |
Concentration | 1.03mg/ml |
Applications | Western Blot. AKT3 is suitable as a control for polyclonal or monoclonal anti-AKT3 in immunological assays. Akt3 recombinant protein is expected to be phosphorylated and in an active state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized. |
Other Names | RAC, PKB, AKT, PKB gamma |
Gene, Accession, CAS # | AKT3, Gene ID: 10000, NCBI: NP_001193658.1, UniProt: Q9Y243 |
Catalog # | 009-001-P24 |
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Order / More Info | AKT3 Human Recombinant Protein from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
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