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| Product Name | Cortisol EIA Kit |
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| Description | The Cortisol EIA kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma and tissue culture media samples. This kit measures total cortisol in extracted samples and in serum and plasma and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol to each well. After an 1 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the cortisol in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers. Competitive EIA (Enzyme Immunoassay). Intra Assay Precision: Three human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Cortisol concentrations were: Sample 1- 1174.3 pg/mL, 6% CV Sample 2- 475.9 pg/mL, 5.6% CV Sample 3- 177.4 pg/mL, 14.7% CV Inter Assay Precision: Three human samples were diluted with Assay Buffer and run in duplicates in ten assays run over multiple days by four operators. The mean and precision of the calculated Cortisol concentrations were: Sample 1- 1188.1 pg/mL, 7.2% CV Sample 2- 508.7 pg/mL, 6.3% CV Sample 3- 199.7 pg/mL, 10.9% CV. Range: 100 - 3200 pg/ml. Sensitivity: 17.3 pg/ml. Time: 90 minutes. |
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| Size | 96 well |
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| Concentration | n/a |
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| Applications | EIA kit used to quantitatively measure cortisol present in samples. RUO |
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| Other Names | StressXpress® (11β)-11,17,21-trihydroxypregn-4-ene-3,20-dione EIA Kit, |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | SKT-201-96 |
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| Order / More Info | Cortisol EIA Kit from STRESSMARQ BIOSCIENCES INC. |
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| Product Specific References | PubMed: 29175482 |
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