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| Product Name | Cas9 Nickase (D10A), GFP, Nuclear Localization Sequence (NLS), Recombinant, Streptococcus pryrogenes, His-tag |
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| Description | The Cas9 nuclease has been modified into a ``nickase'' so that one of its catalytic domains is inactive, resulting in a single stranded nick instead of a double stranded break at the target site on the genomic DNA. In this system not one, but two gRNAs located in close proximity (<20 nucleotides) on opposite strands of the genomic DNA are required in order to create the desired double stranded break. An undesired single stranded nick in the genomic DNA can be quickly repaired by the Homology Directed Repair (HDR) pathway using the other intact strand as the template. The fusion of Cas9 (D10A) Nickase NLS to GFP allows for visual confirmation of transfection as well as subsequent verification of Cas9 clearance from the cells. Cas9 D10A Nickase GFP can also be used for FACS applications and screening. Cas9 D10A Nickase GFP NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein. Source: Recombinant protein corresponding to Streptococcus pyogenes C |
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| Size | 50pmol, 250pmol |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | Cas9 Nickase (D10A), GFP, Nuclear Localization Sequence (NLS), Recombinant, Streptococcus pryrogenes, His-Tag (CRISPR Associated Protein 9) |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 500558 |
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| Order / More Info | Cas9 Nickase (D10A), GFP, Nuclear Localization Sequence (NLS), Recombinant, Streptococcus pryrogenes, His-tag from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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