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| Product Name | Cas9 Nickase (H840A), Recombinant, Streptococcus pryrogenes (CRISPR Associated Protein 9) |
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| Description | Purity ~95% (SDS-PAGE). Cas9 Nuclease Nickase H840A, S. pyogenes, is an RNA guided endonuclease that catalyzes site specific nicking of a single strand of double stranded DNA. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. One concern with the current CRISPR Cas9 technology is the potential off-target effects of the Cas9 nuclease activity. To improve the off-target mutagenic effects of this system, the Cas9 Nickase H840A Protein was developed with a H840A mutation in its HNH-like nuclease domain. This mutant form results in the generation of single strand nick instead of a double stranded break (DSB), like that generated by the Cas9 Nuclease, at the target site. Since a single strand break, or nick, is normally quickly repaired through the homology directed repair pathway using the intact complementary DNA strand as the repair template, off-target |
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| Size | 50pmol, 250pmol |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 500560 |
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| Order / More Info | Cas9 Nickase (H840A), Recombinant, Streptococcus pryrogenes (CRISPR Associated Protein 9) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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