| 产品详情 |
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| Product Name | Cas9, Null, GFP, Nuclear Localization Sequence (NLS), Recombinant, Streptococcus pryrogenes, His-tag |
| Description | The active sites in the Cas9 Null Mutant are inactive, making it a useful control for CRISPR applications. The fusion of Cas9 Null Mutant NLS to GFP allows for visual confirmation of transfection as well as subsequent verification of Cas9 clearance from the cells. Cas9 Null Mutant GFP can also be used for FACS applications and screening. Cas9 Null Mutant GFP NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein. Cas9 Nuclease NLS fused to GFP for visual confirmation of transfection as well as subsequent verification of Cas9 clearance from the cells. Source: Recombinant protein corresponding to Streptococcus pyogenes Cas9, GFP, Null, Nuclear Localization Sequence (NLS), fused to His-Tag, expressed in E. coli. Included Reaction Buffer (10X): Supplied as a liquid in 200mM HEPES, pH 6.5, 50mM MgCl2, 1M NaCl, 1mM EDTA. Reaction Conditions: Use 1X Cas9 Reaction Buffer and incubate at 37°C |
| Size | 50pmol, 250pmol |
| Concentration | n/a |
| Applications | n/a |
| Other Names | Cas9, Null, GFP, Nuclear Localization Sequence (NLS), Recombinant, Streptococcus pryrogenes, His-Tag (CRISPR Associated Protein 9) |
| Gene, Accession, CAS # | n/a |
| Catalog # | 500563 |
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| Order / More Info | Cas9, Null, GFP, Nuclear Localization Sequence (NLS), Recombinant, Streptococcus pryrogenes, His-tag from UNITED STATES BIOLOGICAL |
| Product Specific References | n/a |
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