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PAI-1, Recombinant, Human, aa24-402, His-Tag (Plasminogen activator inhibitor-1, SERPINE1, PAI, PAI1, PLANH1) from UNITED STATES BIOLOGICAL |
| 产品详情 |
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| Product Name | PAI-1, Recombinant, Human, aa24-402, His-Tag (Plasminogen activator inhibitor-1, SERPINE1, PAI, PAI1, PLANH1) |
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| Description | Purity ~95% (SDS-PAGE). Plasminogen activator inhibitor-1(PAI-1), a member of the serine protease inhibitor(serpin) super family. PAI-1 is the principal inhibitor of tissue plasminogen activator and urokinase, the activators of plasminogen and hence fibrinolysis. PAI-1 is mainly produced by the endothelium but is also secreted by other tissue types, such as adipose tissue. Source: Recombinant protein corresponding to aa24-402 from human PAI-1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~45kD (400aa) Biological Activity: Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC, the IC50 value is <56nM. Activity Assay: 1. Prepare a recombinant PAI-1 with various concentrations (2000nM, 1000nM, 500nM, 250nM, 125nM) in assay buffer. (Assay buffer: 50mM Tris-HCl pH 8.0, 0.01% Tween 20) 2. Mix equal volumes of the PAI-1 protein and diluted 2ug/ml uPA 3. Incubate at room temperature for 15 minutes. 4. After dilute incubated reaction mixtures (PAI-1, uPA) by a 5 fold dilu |
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| Size | 100ug, 500ug |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | n/a |
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| Gene, Accession, CAS # | Accession: NP_000593 |
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| Catalog # | 351412 |
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| Order / More Info | PAI-1, Recombinant, Human, aa24-402, His-Tag (Plasminogen activator inhibitor-1, SERPINE1, PAI, PAI1, PLANH1) from UNITED STATES BIOLOGICAL |
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| Product Specific References | n/a |
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