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Antigenic Specificity | AKT1 FITC, internal |
Clone | 5E5.F5.D7 |
Host Species | Mouse |
Reactive Species | human, mouse, rat |
Isotype | IgG2a kappa |
Format | fluorescein (FITC) conjugate |
Size | 50 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot. Anti-AKT1 FITC Antibody is suitable for Flow Cytometry, ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT1 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected. |
Immunogen | Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development. |
Other Names | mouse anti-AKT1 antibody FITC conjugation, fluorescein conjugated mouse anti-AKT-1 antibody, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma |
Gene, Accession # | AKT1, Gene ID: 207, NCBI: NP_001014431.1, UniProt: P31749 |
Catalog # | 200-302-J34 |
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Order / More Info | AKT1 FITC, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
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