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Antigenic Specificity | AKT3 FITC, internal |
Clone | 25F6.F6.D8 |
Host Species | Mouse |
Reactive Species | human, mouse, rat |
Isotype | IgG1 kappa |
Format | fluorescein (FITC) conjugate |
Size | 50 µg |
Concentration | 1.0 mg/mL |
Applications | ELISA, Flow Cytometry, Immunohistochemistry, IF Microscopy, Western Blot. Anti-AKT3 FITC Antibody is suitable for Flow Cytometry, ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from tissue culture by Protein A chromatography. Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined. |
Immunogen | Anti-AKT3 Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography using a synthetic peptide corresponding to internal residues of human AKT3 protein. |
Other Names | Mouse anti-AKT3 antibody FITC conjugation, fluorescein conjugated Mouse anti-AKT 3 antibody, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma |
Gene, Accession # | AKT3, Gene ID: 10000, NCBI: NP_001193658.1, UniProt: Q9Y243 |
Catalog # | 200-302-E75 |
Price | |
Order / More Info | AKT3 FITC, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
Product Specific References | n/a |
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