产品名称 AKT2, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC.
产品货号 K-BR8
产品价格 现货询价,电话:010-67529703
产品规格 1 Pack
产品品牌 ROCKLAND IMMUNOCHEMICALS INC.
产品概述
产品详情
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Antigenic SpecificityAKT2, internal
Clone16C8.E4.G7.E9
Host SpeciesMouse
Reactive Specieshuman
IsotypeIgG2a kappa
FormatProtein A purified
Size1 Pack
Concentration1 mg/ml
ApplicationsELISA, Western Blot. Anti-AKT2 Antibody with the matched secondary are suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT2. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
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DescriptionThis primary and secondary antibody pair has been carefully chosen to give best resutls. Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT2 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT3 and AKT1 has not been determined. HRP secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. This antibody pair is sufficient for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2).
ImmunogenAnti-AKT2 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein. Anti-Mouse IgG HRP secondary antibody was produced by repeated immunizations with Mouse IgG whole molecule
Other NamesAKT 2 antibody pair, AKT-2, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 2 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, primary and secondary antibody pair, Primary+Secondary pair, matched antibody pair
Gene, Accession #AKT2, Gene ID: 10000, NCBI: Q9Y243, UniProt: P31751
Catalog #K-BR8
Price
Order / More InfoAKT2, internal Antibody from ROCKLAND IMMUNOCHEMICALS INC.
Product Specific Referencesn/a
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