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| Product Name | Multispecies IGF-1 ELISA |
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| Description | The biotinylated antibody of the Insulin-like Growth Factor-1 (IGF-1) ELISA Assay kit competes with IGF-I in samples for binding to the antiserum bound to the microtiter plate. After washing to remove excess reagents, a Streptavidin-Peroxidase complex is added, which binds to biotinylated IGF-1. After a washing step, the substrate is added and colour develops. The intensity of the color is inversely related to the quantity of IGF-1 in the sample. The reaction is stopped by addition of stopping solution and the extinction is read at 450 nm. Species Reactivity: human, porcine, bovine and guinea pig. Sample Types: serum, plasma. Range: 1 - 300 ng/ml. Sensitivity: 1 ng/ml. Time: 3.5 hours • Insulin-like Growth Factor-I (IGF-I) is a polypeptide of 70 amino acids. It has a high sequence homology to IGF-II and Insulin. IGF-I is a growth factor for a wide range of cell types. In serum and other body fluids, IGF-I is bound with high affinity to Insulin-like Growth Factor Binding Proteins (IGFBP s). Until today six of the high affinity IGFBP s has been found. In serum the predominant binding protein is IGFBP-3 and more than 95 % of the IGF-I in serum is bound to IGFBP-3. This complex associates with an additional protein, the Acid Labile Subunit, ALS. The binding of IGF-I to IGFBP-3 and other IGFBP s prevents binding to antibodies. Therefore, for ELISA IGF-I has to be extracted before measurement. Another alternative is that samples are acidified to a pH of 2.3. Under these conditions, the IGFBP-3/IGF-I/ALS complex dissociates. If the sample is neutralised in the presence of an excess of IGF-II, the IGFBP s are blocked, and IGF-I remains unbound and is accessible for antibodies. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | IGF-1, Insulin Like growth factor 1, |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | IGF31-K01 |
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| Order / More Info | Multispecies IGF-1 ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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