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| Product Name | NGAL (Stool/Urine) ELISA Assay Kit |
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| Description | This Eagle Biosciences NGAL Stool/Urine ELISA kit is designed, developed and produced for the quantitative measurement of human NGAL in stool samples. The assay utilizes the ``sandwich'' technique with selected antibodies that bind to various epitopes of NGAL. Assay standards, controls and patient samples are added directly to wells of a microtiter plate that is coated with antibody to human NGAL and incubated at room temperature for one hour. The plate is then washed and horseradish peroxidase (HRP) conjugated anti NGAL is added to each well. After an additional incubation period, a ``sandwich'' of solid-phase polyclonal antibody - human NGAL - HRP conjugated antibody'' is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction, which is terminated with an acidic reagent (i.e. ELISA stop solution). The absorbance is then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is directly proportional to the amount of human NGAL in the test sample. A standard curve is generated by plotting the absorbance versus the respective human NGAL concentration for each standard on a point-to-point or 4-parameter curve fitting. The concentration of human NGAL in test samples is determined directly from this standard curve.The validation data of this test were generated by using Fecal Sample Collection Tube (Eagle Biosciences Cat.# CAL35-C50). Each kit contains 50 tubes. A different fecal NGAL test result may be obtained by using a different type of fecal sample collection tube. Sample Types: stool, urine. Range: 1.08 - 97.2 µg/g. Sensitivity: 0.144 µg/g or 0.04 ng/mL. Time: 2 hours • NGAL or neutrophil gelatinase-associated lipocalin also known as Lipocalin-2 (LCN2) or oncogene 24p3 is a protein, which in humans is encoded by the LCN2 gene. NGAL is involved in innate immunity by sequestrating iron that in turn limits bacterial growth. It is expressed in neutrophils and in low levels in the kidney, prostate, and epithelia of the respiratory and alimentary tracts. Studies have shown that NGAL is an early biomarker for ischaemic renal injury after cardiopulmonary bypass. Studies have demonstrated that fecal NGAL is a useful marker for Infammatory Bowel Disease (IBD). Furthermore, NGAL in stool sample extracts is extremely stable. Other studies have demonstrated in Urinary Tract Infected patients, urine NGAL leve is signaifically elevated from normal subjects. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | NGAL, neutrophil gelatinase associated lipocalin, Lipocalin-2, Lipocalin2, 2 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | NGL35-K01 |
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| Order / More Info | NGAL (Stool/Urine) ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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