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| Product Name | CoproELISA C. difficile Tox A/B |
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| Description | CoproELISA C. difficile ToxA/B is an enzyme immunoassay for the detection of toxin A and toxin B in human feces. Break-apart microwells are coated with C. difficile toxin-specific polyclonal antibodies. A set of horseradish peroxidase (HRP) conjugated polyclonal anti-toxin A and anti-toxin B antibodies are added to the antibody-coated microwells. Fecal samples are diluted in sample diluent and added to the microwells. In this step C. difficile toxins are specifically marked by the HRP conjugated antibodies and immobilized by the coated antibodies. Unbound conjugate is removed by washing. Upon the addition of TMB-substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced substrate. Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620 nm. The absorbance is proportional to the level of C. difficile toxins in the sample. Sample Types: stool. Range: Cut-Off. Sensitivity: Cut-Off. Time: 2 hours • The gram-positive anaerobic bacillus Clostridium difficile is the leading causative agent of antibiotic-associated diarrhea and pseudomembranous colitis. This pathogen is capable of causing disease that could be severe or fatal if not diagnosed on time and treated. Exposure to antibiotics is the major risk factor for C. difficile infection. Infection can develop if the normal gastrointestinal flora is disrupted by antibiotic therapy and a person acquires toxin-producing C. difficile, typically via the fecal-oral route. C. difficile's key virulence factors are toxin A and toxin B. These toxins show high sequence and functional homology. Toxin A has been described as a tissue damaging enterotoxin which attracts neutrophils and monocytes and toxin B as a potent cytotoxin that degrades the colonic epithelial cells. Most virulent strains produce both toxins, however, strains that are toxin A negative and toxin B positive are also capable of causing. Immunoassay detection of toxin A and toxin B in stool specimen is commonly used as a diagnostic aid. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | C. difficile Tox A/B, stool, fecal |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 794-01 |
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| Order / More Info | CoproELISA C. difficile Tox A/B from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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