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| Product Name | Dengue virus NS1 ELISA Kit |
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| Description | This assay employs the sandwich enzyme immunoassay technique for the detection of Dengue virus NS1 in serum, plasma and cell culture supernatant samples. An antibody specific for DENV NS1 has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any DENV NS1 present is bound on the plate. After washing away any unbound substances, a Horseradish Peroxidase (HRP) conjugated primary antibody binds to DENV NS1 is added to each well and incubates. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of total DENV NS1 bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm. The concentration of total DENV NS1 in the sample is then determined by comparing the O.D of samples to the standard curve. Sample Types: serum, plasma and cell culture supernatants. Range: 1.56 - 100 ng/ml. Sensitivity: 0.5 ng/ml. Time: 2.5 hours • Dengue virus NS1 protein is a nonstructural protein which could be secreted and have been developed as diagnostic biomarker for early detection. There are several forms of NS1 including monomer, dimer, and hexamer during infection. Dimeric NS1 can be anchored to cell membranes with glycosyl-phosphatidylinositol (GPI). Hexameric NS1 can be secreted and detected in patients' blood samples (up to 50 µg/mL) or infected cell supernatants (various from ng/mL to µg/mL depend on serotypes and strains). Studies have shown that NS1 could interfere complement activity and prothrombin activation. In addition, NS1 could elicit antibodies which cross-react with host antigens including coagulation factors and molecules expressed in endothelial cells and platelets through molecular mimic. Dengue virus (DENV) non-structural protein 1 (NS1) is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome (By similarity). |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Dengue Virus NS1 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ARG81357 |
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| Order / More Info | Dengue virus NS1 ELISA Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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