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| Product Name | Human HMGB1 ELISA Kit |
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| Description | This assay employs the sandwich enzyme immunoassay technique for the detection of Human HMGB1 in plasma and cell culture supernatants samples. An antibody specific for HMGB1 has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and then a Horseradish Peroxidase (HRP) conjugated primary antibody binds to HMGB1 is added to each well and incubates. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of total HMGB1 bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm. The concentration of total HMGB1 in the sample is then determined by comparing the O.D of samples to the standard curve. Sample Types: plasma, cell culture supernatants. Range: 0.3125-20 ng/ml. Sensitivity: 0.3 ng/ml. Time: Overnight • Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be a universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | HMGB1 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ARG81185 |
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| Order / More Info | Human HMGB1 ELISA Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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