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Product Name | Human MMP-9 ELISA KIT |
Description | The Eagle Biosciences Human Matrix Metalloproteinase 9 (MMP-9) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for MMP-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-9 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for MMP-9 is added to the wells and binds to the combination of capture antibody-MMP-9 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of MMP-9 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven MMP-9 standard dilutions and MMP-9 sample concentration determined. Sample Types: serum, plasma, cell culture. Range: 62.5 - 2000 pg/ml. Sensitivity: 31 pg/ml. Time: 3.5 hours • The Eagle Biosciences Human Matrix Metalloproteinase 9 (MMP-9) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Matrix Metalloproteinase 9 (MMP-9) concentrations in cell culture supernates, serum, and plasma. The Eagle Biosciences Human Matrix Metalloproteinase 9 (MMP-9) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures. |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Matrix Metalloproteinase 9, MMP-9, gelatinase B |
Gene, Accession, CAS # | n/a |
Catalog # | MM931-K01 |
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Order / More Info | Human MMP-9 ELISA KIT from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |
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